Regulatory

Part:BBa_K3245017:Design

Designed by: Jianyi Hu   Group: iGEM19_Fudan   (2019-10-19)


Enhanced QS promoter regulated by luxR-AHL complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We substituted the -35 to -10 region of the original promoter luxpR-HS with the one of J23100 / J23106, a strong constitutive promoter.


Source

Derivated from the luxpR-HS promoter designed by 2018 Tsinghua team.

References